CLL

Chronic lymphocytic leukemia-FISH

Probes

6q- [SEC63 (6q21), MYB (6q23)

 ATM (11q22.3) | p53 (17p13.1)

 Trisomy 12 (Cen 12) | 13q-/-13 (13q14, 13q34)

CCND1/IgH t(11;14)

Probes may be ordered separately (except 12 and 13 are tested together, and ATM is tested with p53)
Disease(s): Chronic lymphocytic leukemia

Note: This test was previously called CLL FISH Panel (non-New York). It is now New York state-approved and available to all clients

Clinical Significance

The CLL FISH panel is used for the detection of chromosome aberrations observed in chronic lymphocytic leukemia/small lymphocytic lymphoma, which are useful in prognosis and prediction of time to treatment in CLL patients when used in combination with other clinical and diagnostic findings. CCND1/IgH is used to rule out mantle cell leukemia/lymphoma

Specimen Requirements

  • Bone Marrow Aspirate: 1-2 mL sodium heparin tube. EDTA tube is acceptable

Peripheral Blood: 2-5 mL sodium heparin tube. EDTA tube is acceptable

Fresh, Unfixed Tissue: Tissue in RPMI

Bone Marrow/ Peripheral Blood Smear or Fresh Tissue Touch Preparation Slides: minimum 4 slides labeled with specimen type

Fluids: Equal parts RPMI to specimen volume

Fixed Cell Suspension: A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol: Glacial Acetic Acid

Paraffin Block: Send paraffin block. Also send circled H&E slide for tech-only (required). Note: Not approved for NYS

samples

Note: Please exclude biopsy needles, blades, and other foreign objects from transport tubes. These can compromise specimen viability and yield, and create hazards for employees

Storage & Transportation

Use cold pack for transport, making sure cold pack is not in direct contact with specimen. For fresh samples: ship same day as drawn whenever possible; specimens <72 hours old preferred

ALL

Acute lymphoblastic leukemia

: Probes

TCF3/PBX1 (E2A/PBX1) t(1;19)

 Trisomy or Tetrasomy 4, 6, 10, 17 (Cen 4, Cen 6, Cen 10, Cen 17)

MYC (8q24)

| BCR/ABL1/ASS1 t(9;22)

MLL (11q23) | IgH (14q32)
Disease(s): Acute lymphoblastic (lymphocytic) leukemia (B-cell ALL), B lymphoblastic lymphoma (LBL), adult Probes may be ordered separately except Centromeres 4 and 17 are paired, and Centromeres 6 and 10 are paired


Note: STAT processing is available by request for BCR-ABL1. Note STAT along with MD contact name and phone number to receive STAT results
Note: CDKN2A (p16) Deletion FISH is also available and may be ordered separately. See details here

Clinical Significance

The ALL Adult FISH Panel is used for the detection of recurrent chromosome abnormalities observed in adults with ALL of B-cell lineage and B lymphoblastic lymphoma (LBL). Identification of specific abnormalities helps predict disease aggressiveness and response to therapy. This panel differs from the ALL Pediatric FISH Panel in that this panel excludes probes for ETV6/RUNX1 t(12;21).