CML/MPD panel | FISH
Test Details | Detects common gene rearrangements associated with CML: BCR/ABL t(9;21), FIP1L1/PDGFRA CHIC2/del 4q12 |
Relevant condition(s) | CML |
Methodology | Fluorescence in-situ hybridization |
Performed | Monday – Saturday |
Turnaround | 3 – 5 days |
Specimen requirements | Suspension FISH on Bone marrow, Bone core, Lymph node or Peripheral Blood ONLY |
Sample collection | In sterile 15 mL sodium heparin (green-top) vacutainer tube collect 1 – 2 mLs bone marrow; 5 – 10 mLs blood; 0.5 – 1.0 cm2 biopsy (bone core; lymph node); invert tube immediately after collection to prevent clots |
Minimum sample volume | bone marrow; 2 mL blood |
Storage/Transport Conditions | Room temperature |
Unacceptable Conditions | Frozen specimens, clotted or hemolyzed specimens, inappropriate tube type, paraffin sample; Will not be accepted after three days |
Special Instructions | Refrigerate if sample cannot be delivered within 24 hours |
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